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KMID : 1007520030120020137
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Food Science and Biotechnology
2003 Volume.12 No. 2 p.137 ~ p.141
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Accumulation of Isoleucine Intermediates in the Isoleucine Producing Recombinant of Corynebacterium lactofermentum ATCC 21799
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An Gil-Hwan
ANTHONY J. SINSKEY
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Abstract
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Among the five consecutive enzymes in the anabolic pathway from threonine to isleucine in Corynebacterium threonine dehydratase and acetohydroxy acid synthase are feedback inhibited by isoleucine. Increased activation of threonine dehydratase in C. lactofermentum ATCC 21799 resulted in accumulation of 2-aceto-2-hydroxybutyrate (substrate of dihydroxy acid synthase) and 2,3-dihydroxy-3-methylvalerate (substrate of dihydroxy acid dehydratase), but not α-keto-butyrate (product of threonine dehydratase and substrate of acetohydroxy acid synthase). This result suggests that dihydroxy acid synthase and dihydroxy acid dehydratase must be amplified to obtain increased isoleucine titer.
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KEYWORD
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